Abstract: Objective  To evaluate the diagnostic accuracy of loop-mediated isothermal amplification (LAMP) in detecting Mycobacterium tuberculosis in clinical samples.  Methods A fully recursive literature search was conducted by searching PubMed, Embase, Web of Science，Cochrane Library, CNKI，Wanfang and VIP databases. QUADAS criteria were used to evaluate the quality of the included studies, and Meta-Disc software was used to analyze data collected from the included studies. Sensitivity, specificity and summary receiver operator characteristic curve （SROC）were chosen as the indicators to assess the diagnostic accuracy of every individual diagnostic test. Heterogeneity between the individual studies was analysed by Meta regression.  Results  A total of 16 articles which including 18 studies were enrolled in the assay. Meta-analysis showed that the pooled sensitivity of LAMP was 0.91（95%CI 0.90-0.93）, the pooled specificity was 0.97（95%CI 0.96-0.97）, the pooled diagnostic odds (DOR) was 283.02（95%CI 157.37-509.00），the area below SROC curve(AUC) was 0.9829.Meta regression analysis showed that the studies with result interpretation by UV light have 5.66(RDOR=5.66,95%CI：1.32-24.22, P=0.0225) RDOR  than the studies with result interpretation by naked eyes.  Conclusion  The conducted researches confirm that LAMP assay is a highly specific test for the detection of Mycobacterium tuberculosis from clinical samples and has a potential in improving the diagnostic accuracy of in detecting Mycobacterium tuberculosis.

Key words: Mycobacterium tuberculosis, Nucleic acid amplication techniques, Sensitivity and specificity